Protein, Fat and sugar Residue Test (RRS 14-41210)

Structure and test description of RRS protein, fat, and sugar residue test on surfaces

The RRS protein, fat, and sugar residue test consists of a swab for sampling surfaces or tools and two plastic tubes A (solid phase) and B (liquid phase) containing a reagent to detect contamination.

The RRS protein test is a very sensitive, accurate, rapid, and simple test to detect small amounts of protein, fat, and sugar on the endoscopes, surgical instruments, and also surfaces of washing or disinfectant machines and ultrasonic cleaners. In this method, the colorless liquid turns purple in the presence of contamination. The intensity of the purple color depends on the amount of protein in the sample. Compared to other methods, it is a more accurate method because all the peptide bonds participate in the formation of the dye.

Purpose of use

  • Detection of low protein concentrations (0.5 µg/ml)
  • Easy-to-interpret colour change
  • Easy to use

Caution: Gloves must be used while using the protein kit to avoid protein contamination by the skin.

Instructions for Use of the RRS Test

First, add the clear liquid of tube B into tube A (Figure 1). Shake for 30 to 60 seconds until the liquid in tube B is completely dissolved in solution A and green color appears (Figure 2).

Take the swab out of its cover.

Rub the swab firmly over the desired surface if the surface is wet; and if the surface is dry, first, moisturize the swab with sterile or distilled water and then rub it firmly over the desired surface.

Unscrew the reagent tube and swirl the swab in the reagent (Figure 4). Do not remove the swab from the solution to see a better result.

One of the three-room (20-25 ° C) temperatures, 37 and 57 ° C, can be selected to check the result. Check the test result according to the times shown in the table below.

Minimum and maximum color change time of the Protein, Fat and sugar Residue Test (RRS 14-41210)

If there is less pollution, over time, the color of the solution changes from green to gray (low pollution level), then pink-lilac (medium pollution level), and finally purple (high pollution level). If the green color of the solution does not change, it can be said that the desired surface is free of protein.

Record the results of the RRS test detection in the RRS CSSD Documentation NoteBook.


Keep the RRS test in the fridge or in the 2-8 °C, away from sunlight, high temperature, disinfectants, and chemical agents.

Be sure to use gloves when opening the tube cap.


18 months after the production


Discard indicators in accordance with your country’s health and safety regulations (elimination instructions).

Download Catalog of RRS Test

Please Check out file at the following link

Protein Residue Detection Test on surfaces (RRS 14-41310)

Protein Test (Indicator of Monitoring the Washing Process) (RRS 14-41110)

Steam Chemical Indicator class 6 (RRS 14-11660)

Steam Biological Indicator 10^5 (RRS 14-21110)